FPA: Fiber Photometry Analyzer.

Analyze Photometry Data with Ease - No coding Required.

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FPA: Overview.

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The Problem

Fiber photometry, like many modern neuroscience tools, produces huge datasets that are challenging to handle and analyze without coding skills.

Too Much Data to Process

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Lack of Appropriate Analysis Tools

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Existing options are limited, often missing key features for different experimental designs and still largely requiring coding knowledge to implement.

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How do we address it?

Fiber Photometry Data Analysis Tool

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We’ve developed an online software that helps you to analyze and explore vast photometry data sets very quickly.

FPA makes high-quality photometry analysis accessible to everyone, no coding or data science expertise required.

No-code Tool

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What do we do differently?

Embrace Cutting-edge Technology

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At Metofico, we harness cutting-edge methods from across industries to push neuroscience forward.

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Continuous Improvement

We continuously refine our platform and tools based on customer feedback. Users can easily request new features to be added to the platform.

Our tools are designed to work together, for example behavioral timestamps from TOMB-AI can be used directly in FPA.

Module Integration

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Relevant Publications

Learn More:

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Input & Preprocessing

    • Batch import supporting several file formats.

    • Up to 4 light channels per animal.

  • Effortlessly eliminate large artefacts from your data.

  • Enhance data clarity using digital filters.

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Study System

    • Conveniently segment your data with multiple events.

    • Define events as single points or as time-bounded events for precise analysis.

    • Easily organize subjects into groups and subgroups.

    • Perform individual/group analyses.

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Analysis & Visualization

  • Offers several normalization methods to accommodate a variety of experimental designs, including ΔF/F, isosbestic fitting, and z-score.

  • We offer a range of analysis tools to assist with data exploration, including pre-post baseline comparisons, z-score analysis, and peak detection.

  • Built-in plotting features to quickly explore your data including a variety of line plots, bar plots and heat maps.

FPA: Benefits.

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Efficiency

FPA allows for fast processing of vast data sets without writing a single line of code, thus accelerating scientific output.

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Reliability

By leveraging FPA, you ensure high-quality data processing and analysis powered by the latest published algorithms.

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Repeatability

FPA ensures precise, repeatable analysis - same data, same parameters, same results every time, regardless of skill level.

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Frequently Asked Questions (FAQ’s).

  • Fiber photometry is a powerful technique for monitoring population-level calcium dynamics in awake, behaving animals. It involves the implantation of a fiber-optic cannula, which delivers excitation light to neurons that express genetically encoded calcium indicators and collects fluorescence signals that reflect calcium fluctuations. These calcium transients serve as indirect measures of neuronal activity, allowing researchers to investigate correlations between neural dynamics and behavioral states.

  • Our modules are fully no-code, meaning you can use them from start to finish without writing a single line of code. This is crucial because programming skills are often a barrier to efficient and advanced data analysis. By eliminating this requirement, we ensure that high-quality data analysis is accessible to all researchers, regardless of coding experience.

  • Although open-source software is technically free, the significant time and effort required for implementation and customization can lead to substantial hidden costs. Moreover, once deployed, these tools often suffer from poor interface design and a lack of critical features, which hampers usability. In contrast, FPA provides a robust no-code tool accompanied by expert guidance.

  • The software was initially designed to be compatible with doric hardware systems, which is what ourselves and our collaborators have primarily used. However, we can easily adapt the software to work with other systems by modifying the data extraction and organization process.

  • FPA is designed to accept Excel file inputs, as this is the most common output format from fiber photometry hardware systems. However, we can modify our software if other data input formats are required.


  • The inbuilt software systems often lack the usability and features needed to effectively process and analyze fiber photometry data sets. This is based on both our experience and that of our collaborators.

  • At Metofico we constantly update our software based on customer feedback and market demand, keeping our solutions up-to-date with advancements in the field.

Get In Touch.

To find out more about our products and services please contact us at info@metofico.com, via social media or fill out the form.

We offer free demos and trials of our software, allowing you to explore how our solutions can enhance your specific research or operational goals.